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Subcutaneous (SubQ) injection is the better route for most 5-Amino-1MQ protocols due to consistent absorption kinetics, lower tissue trauma across repeated dosing, and easier self-administration without specialized technique

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HUVECs were harvested by trypsinEDTA, plated onto the gel at 1.5 10 5 cells per well with serum-free medium (Invitrogen) containing EGF (Invitrogen), and incubated overnight