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Dual luciferase reporter activity assay The dual luciferase assay was conducted following established protocols (Promega, Madison, USA)

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Future studies on flavonoids in coconut products should focus on comparative analyses of different cultivars, comparisons with other fruits, optimization of extraction methods, in vivo studies to assess health benefits, investigations of synergistic effects with other nutrients, and examinations of developmental dynamics to fully understand their antioxidant properties and potential applications