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Carbamidomethylation of cysteine was specified as a fixed modification, and acetylation at the protein N-terminus and oxidation of methionine were set as variable modifications

They proved to work also with Phi and Tau GSTs, the latter binding porphyrin intermediates and fatty acids (Dixon et al., 2008, 2011

The rate-limiting step in GSH synthesis is the availability of cysteine, which is transported into the brain from the blood via the cystine/glutamate antiporter system xc-