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This process enables the endometrium to acquire a receptive phenotype, promoting embryo implantation and pregnancy maintenance by providing a nutritive and immunoprivileged environment ( Figure 1 Concurrently, during decidualization, the differentiation of stromal cells is accompanied by significant metabolic changes, with glucose serving as a primary energy source ( Aberrant glucose metabolism, characterized by dysregulated glucose uptake or glycolysis in human endometrial stromal cells (hESCs), contributes to decidualization deficiencies and subsequent embryo implantation failure ( Figure 1 )

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The lysate was centrifuged for 30 min at 13000 rpm, and the supernatant was applied to GST trap Glutathione Sepharose 4B (GE17-0756-01, Merck) over night at 4 C

GSH-dependent activation releases Cu + to engage lipoylated DLAT and simultaneously unmasks glucose oxidase activity, inducing nutrient deprivation and triggering cuproptosis 216

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Vitamin B12 is an essential nutrient that plays a critical role in the production of red blood cells, the maintenance of the nervous system and DNA synthesis