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doi: 10.1124/jpet.104.066126

The analyses of 4MSOB ITC and its conjugates were conducted using an Agilent HP1260 Series instrument coupled to an API5000 tandem mass spectrometer (AB Sciex, Darmstadt, Germany) by loading extracted samples onto an Agilent Zorbax Eclipse XDB-C18 column (50 4.6 mm 1.8 m, Agilent Technologies, Waldbronn, Germany) with mobile phase A (0.05% formic acid in milliQ water) and mobile phase B (acetonitrile)

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In simple terms, the molecules become less likely to stick together, which can help the peptide move into a clearer and more uniform solution

BMC Public Health 2012;12:394404

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