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Moreover, ALA exerts antioxidant effects via a dual mechanism: (i) rapid redox-cycling between its disulfide (ALA) and dihydrolipoate (DHLA) forms, which directly quenches ROS without transcriptional activation, and (ii) secondary upregulation of the Nrf2 pathway and suppression of NF-B nuclear translocation (Ghibu et al., 2009)

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Subsequently, the membranes were incubated with one of the secondary antibodies: goat-anti-rabbit-horseradish peroxidase (HRP) (1:20000

The supernatant was transferred to a new Eppendorf tube for derivatization with 3-nitrophenylhydrazine (25 mM), N-(3-Dimethylaminopropyl)-N-ethylcarboimiide hydrochloride (EDC) (25 mM) and pridine (7%, w/w)

Reported rationale: peak GI effect is concentrated to a single day rather than smeared across the week

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