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Briefly, protein extracts were prepared by homogenizing with a Polytron homogenizer (KINEMATICA AG, Switzerland) in lysis buffer containing: 20 mM HEPES (pH 7.9), 25% v/v glycerol, 0.42 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 0.5% v/v Nonidet P-40, 1 mM DTT, 1 mM Naf, 1 mM Na 3 VO 4 , and 1 complete protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany)

Investigations have revealed that the orphan nuclear receptor NR4A1 is pivotal in mediating metformins protective effects against endothelial dysfunction induced by high glucose levels

Medesan C, Cianga P, Mummert M, Stanescu D, Ghetie V, Ward ES

Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice

Chan SSM, Luben R, et al