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In all, 30 l of PCR reaction mixture was comprised of genomic DNA samples (100 ng), 200 mol l 1 dNTPs, 1.5 mM MgCl 2 , 1 Taq polymerase buffer, 100 pmol of each primer and 0.5 U of Taq DNA polymerase (Amersham, Paris, France)

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