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After removing the DEPC-water and adding 15 mL Triethanolamine-acetic anhydride solution for 10 min at RT, slides were washed in DEPC-PBS at room temperature for 5 min and pre-hybridize in hybridization solution (50% Formamide, 5 SSC, 0.3 mg/mL Yeast tRNA, 100 g/mL Heparin, 1 Denhardts Solution, 0.1% Tween 20, 0.1% CHAPS, 5 mM EDTA) for 1-4 h in a 65 C water bath

[PMID: 19596934]

In clinics it is used for skin brightening and antioxidant support

The antidepressant fluoxetine (Prozac) modulates estrogen signaling in the uterus and alters estrous cycles in mice

most of which can not be found in any other natural source

It has two main pathological characteristics, one is senile plaques mainly composed by amyloid- (A) peptides which is resulted from aberrant amyloid precursor protein (APP) cleavage, and the other is neurofibrillary tangles (NFTs) basically consisting of paired helical filaments (PHFs) which are derived from hyperphosphorylated tau proteins (Tseng et al., 2002)