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Reactions were carried out at 37 C for 20 min with a total volume of 20 L, and terminated by heating at 95 C for 5 min

Ann N Y Acad Sci 2004;1033:117-31

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In some studies, telomere length rose by an average of 33.3%, though results varied between individuals, likely due to differences in baseline telomerase activity, cellular age, and other metabolic factors [4, 14]