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For the construction of scFv-A36 mutants, a DNA fragment, including the CDR1 and CDR3 regions of the heavy chain of A36, was amplified using PCR and the following degenerate primers: HindIII (underlined) sense linker primer (5-GCA AAGCTT CTGGCTTCNNNNNNNNNNNNNNNNNNNNNNNNTGGGTGAAGCAGAGGCCTGCACAGG-3) and BstEII (double underlined) antisense linker primer (5-GGAGAC GGTGACC GTGGTCCCTTGGCCCCANNNNNNNNNNNNNNNNNNNNNNNNAGCACAGTAATAGACGGCAGTGTCCTCAG-3), in which N is A, C, G, or T (equimolar)

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how to get rid of labial melanotic macule Lip Pigmentation Treatment Singapore Dark spots on lips: 13
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