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Simultaneously, MGS1 cells were seeded at 2 10 cells per 10 cm dish and cultured in parallel for preparation of the macrophage treatments

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The following monoclonal antibodies (mAbs) or polyclonal Abs (pAbs) were used in the present study: rat anti-mouse F4/80 mAb (Dainippon Pharmaceutical Company, Osaka, Japan), rabbit anti-myeloperoxidase (MPO) pAbs (Neomarkers, Fremont, CA), rabbit anti-APAP pAbs (Biogenesis Inc., Kingston, NH), rabbit anti-CYP1A2 pAbs, rabbit anti-CYP3A pAbs, rabbit anti- -actin pAbs, rabbit anti-mouse NF- B p65 pAbs (Santa Cruz Biotechnology, Inc, Santa Cruz, CA), rabbit anti-CYP2E1 pAbs (Daiichi, Pure Chemicals, Tokyo, Japan), and rabbit anti-single-strand DNA (ssDNA) pAbs (Dako, Kyoto, Japan)