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Description

Realtime PCR was performed using TaqMan Universal Master Mix II, no UNG (Applied Biosystems PN 4440041) according to the protocol, using 1.33 l of undiluted cDNA per reaction and RNAspecific Taqman hydrolysis probes

While an arm lift or brachioplasty may still be necessary, the awake nature of the procedure sets it apart by eliminating the requirement for general anesthesia