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This is primarily due to the lack of universal standards and norms in addition to the assay complexity and high costs (170173)

These enzymes include sirtuins, poly(ADP-ribose) polymerase (PARP) protein family, and cyclic ADP-ribose (cADPR) synthases

In the hospitalization, 3 patients died

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The PCR conditions were adjusted according to the kit protocol as follows: reverse transcription for 10 min at 45 C, Polymerase activation for 2 min at 95 C, denaturation (40 cycles) for 5 s at 95 C, and annealing/extension for 20 s at 60 C