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For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

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GSK-3 inhibitors improve the cardiac function of AnkB-deficient ACM mouse models, and the use of these inhibitors may become a new prevention and treatment strategy for ACM