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Briefly, mice were decapitated and the brain was rapidly extracted in oxygenated (95% O2 and 5% CO2) and ice-cold sucrose-based artificial cerebrospinal fluid (sucrose-aCSF) at pH 7.4 and 300 mOsm containing: 185 mM sucrose (Sigma Aldrich S9378), 25 mM NaHCO 3 (Thermo Fisher, BP328), 2.5 mM KCl (Millipore Sigma, P9333), 25 mM glucose (Millipore Sigma, G8270), 1.25 mM NaH 2 PO 4 (Sigma Aldrich S8282), 10 mM MgCl 2 (Millipore Sigma, M9272), and 0.5 mM CaCl 2 (Millipore Sigma, 223506)

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It has sufficient composition of all essential amino acids that meet standard dietary recommendations (Thevarajah et al

Role in cell proliferation: Different studies indicate that glutathione promotes proliferation of different types of cells such as human fibroblast cells, lymphocyte, hepatocytes, mouse bone marrow cells and intestinal epithelial cells

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