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Top-down MS alternative: ETD or EThcD fragmentation of the intact small fragment gives the N-terminal sequence in ~30 minutes if you have access to an Orbitrap with ETD

And so that probably more often than not is a limiting factor

Zeel Gandhi Jordan Anthony is a Registered Dietitian Nutritionist with over 6 years of experience

5e,f) respectively

So, what about endo and B12 specifically

Further scrutiny of the locus revealed that both copy number, location and direction of individual GSTM1 genes are shuffled within the same locus (Fig