measuring glutathione in serum Effects of the M. sylvestris extract on the reduced Glutathione GSH/GSSG Assay Kit, MAK440,

To introduce the C-terminal Azo-tag into the coding region for the sfGFP reporter protein, two Gly codons and the amber stop codon were inserted between the C-terminal Strep -tag II and the final ochre stop codon, thus placing Pap as the last encoded residue in the reading frame, and this gene cassette was subcloned on pSB19 between the Xba I and Hin dIII restriction sites

Non-specifically bound, contaminating proteins were removed by washing with equilibration buffer

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Furthermore, reduced mitochondrial mobility was indicated by the downregulation of RHOT1 and RHOT2 (Zaninello and Bean 2023), while proteins related to autophagy (SQSTM1, MAP1LC3B, ATG2 A) and mitophagy (FUNDC2) (Fan et al