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Both free HSP and HSP-HA-SLN were well tolerated at lower concentrations relevant to topical antioxidant therapy, keeping cell viability above 90%
The membranes were washed with Tris-buffered saline containing 0.2% Tween 20 and incubated with the secondary antibody horseradish peroxidase-conjugated (rabbit anti-sheep, 14000 to SOD-1 and goat anti-mouse, 13000 to catalase)
Lactoferricin enhances BMP7-stimulated anabolic pathways in intervertebral disc cells
Int J Environ Anal Chem 94(7):668678