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Then, it was centrifuged (for 15 min at 3500 rpm) and tris buffer and 5, 5-dithiobis-(2-nitrobenzoic acid) (DTNB) solution was added to the mentioned solution

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The trial achieved its primary end point, demonstrating a statistically significant mean absolute difference in FVC at 12 months between the cyclophosphamide and the placebo arm of 2.53% in favor of the cyclophosphamide arm of the trial