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Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies for 1 h at room temperature

Again, this step is limited by the smaller stomach size and even more limited in the bypass surgery patients since the duodenum is bypassed

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Furthermore, Y358 is locked in place by E383 and K436, an electrostatic pair conserved in OCTs and OATs 34 that is involved in the coordination and release of substrates within the pocket 29 (Supplementary Fig