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PMID 26288227

1-MNA and S-(5-adenosyl)-L-methionine (SAH) were obtained from Cayman Chemical Company (Ann Arbor, Mich., USA) 5.1 Parallel Artificial Membrane Permeability Assay (PAMPA)

A naturally occurring coenzyme found in nearly every cell, NAD+ plays a key role in energy production and overall balance

this, in turn, leads to accentuated elevations in blood concentrations of tHcy and MMA, and exacerbation of neurological sequelae due to vitamin B-12 deficiency in the brain and peripheral nervous system To test these and other predictions, in addition to human studies, it will be important to develop better animal models with closer similarity to the physiologic and clinical features of vitamin B-12 deficiency than rodent models currently provide