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mice were sacrificed 90 min later using an overdose of sodium pentobarbital (Euthasol) and perfused first with 0.1 M PBS (pH 7.4) then with 4% paraformaldehyde (Jassim and Inman, 2019)

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2.1.3 Microglia Microglia constitute specialized immune sentinels of the central nervous system that exhibit similar plasticity to macrophages, transitioning between inflammatory and anti-inflammatory phenotypes

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B12: Energy & Fatigue Reduction B12 is a critical cofactor in mitochondrial energy production