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If the B12 ingested was part of a multivitamin, then the list of potential sources for a reaction becomes quite formidable

For example, leaving plastic bottles in a hot car or reusing single-use bottles many times may not be the best habit

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For saturation binding assays, cell lysates (100 L, 25 g of protein for HEK-293 cells and 1020 g of protein for INS-1E cells, quantified by the Bradford Assay) were incubated with the indicated concentrations of [ 3 H]RX821002 (90 min, room temperature)

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