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1997), which argues that both GA and ALT reactions contribute to the glutamate pool

Compound 5 was isolated by silica gel column chromatography using a dichloromethane/methanol (DCM:MeOH = 20:1, V/V) eluent (yield: 61%)

Intracellular ROS can be detected with the DCFH-DA reporter that is converted to fluorescent 2,7-dichlorofluorescein (DCF) upon exposure to ROS (Jiang et al, 2021)