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(5)Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (5) Preparation of Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (4)Fmoc-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Asp(otBu)-OH1.2gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Ala-Gly-Leu-Val-WangResin of step (4), add decapping reagent (DMF solution of 20% piperidine), react at normal temperature for 5 minutes, drain, add decapping agent normal temperature and react for 20 minutes, use Wash 3 times with isopropanol, 3 times with DMF, 2 times with distilled DMF, drain, add Fmoc-Asp(otBu)-OH1.2g, DIEA0.4ml, TBTU0.56g, HOBT0.2g, DMF10ml at room temperature Reacted for 1.5 hours, dried with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with re-distilled DMF, and dried to obtain Fmoc-Asp(otBu)-Ala-Gly-Leu-Val- Wang Resin

Theres an evolution in the lore of Dark Souls here, from bells developing from a minor theme to a major one, which coincidentally seems to predict their centrality to the apparently unconnected future of Bloodborne

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