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Peptide-free crystals were also obtained using a counter-diffusion crystallisation method 40 under a microgravity environment in the Japanese Experimental Module Kibo at the International Space Station (ISS) 41

The inoculum for infection was prepared by diluting stock vials as described previously ( Aerosol Infection of Mice, Treatment, and Bacterial CFU Assay The diabetic C57BL db/db mice of 68 weeks old were purchased from Jackson Laboratories (Bar Harbor, ME, United States)

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