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Crosslinking was quenched with ammonium bicarbonate to a final concentration of 20mM before the addition of 20uL alkaline phosphatase (37C, 30min, shaking at 500rpm) to 200g of sample

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The subcellular distribution of the S114A mutant favored a more pronounced nuclear localization with significantly less fluorescence in the cytosol compared to the full-length transgene (Fig

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