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After incubation of the secondary antibody, Alexa Flour 488-conjugated goat anti-rabbit IgG (Thermo Fisher), cells were resuspended in 200 l PBS/1% BSA and measured on a BD FACS Canto II flow cytometer (BD Bioscience)

In vitro studies demonstrated cytotoxic activity in HepG2 cells and human liver cells L-02, leading to cell death, and demonstrated in in vivo studies that flavokavain B is responsible for the potentiation of kava hepatotoxicity upon herbdrug interaction in C57BL/6 mice

Research observations may include increased appetite, energy fluctuations and occasional discomfort

With a formula containing Glutathione, it helps whiten skin from deep within, prevents aging and effectively protects the skin from the harmful effects of UV rays

doi:10.1056/NEJMra2402527

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