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Ipamorelin : 2nd-generation GHRP, highly selective for GHS-R1a, no significant impact on cortisol or prolactin the current darling of modern protocols GHRP-6 : first generation, potent but triggers marked hunger (ghrelin-like action on the arcuate nucleus) and slight prolactin/cortisol elevation Hexarelin : most potent on acute GH release, but rapid GHS-R1a desensitization after prolonged use and more pronounced cortisol elevation Key point: GHRPs add an amplifying signal that potentiates GHRH, but chronic monotherapy triggers receptor down-regulation

The SYBR Green Pro Taq HS premix qPCR assay kit from Accurate Biotechnology Co., Ltd was used for reverse transcription of total RNA

Concentrated fractions were then incubated with 20mM GSSG (Thermo J63715.06) and PMab1 Fv-clasp at a 1:1.5 molar ratio for 30 min at room temperature