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One of the important members of the vitamin B complex is vitamin B12, the only vitamin containing a metallic element, namely cobalt

It binds to vitamin B12 so that the vitamin can be absorbed into the blood

In addition, if central venous access is used, this carries its own risks

Cell culture Cell lines were maintained in RPMI 1640 (Thermo Fisher, 11875119) with 5% FBS (Sigma-Aldrich, SH30396.03) and 1% penicillin and streptomycin (Thermo Fisher, 15070063)

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[2] : 71 Although over 500 amino acids exist in nature, by far the most important are the 22 -amino acids incorporated into proteins