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J Clin Investig 99:846854

Hammond BR Jr, Wooten BR, Snodderly DM

COS-7 cells, seeded onto 10-mm dishes, were lysed with a lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 mM EDTA and protease inhibitor cocktail) by pipetting and sonication

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