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Clean the rubber tops of both the MOTS-c vial and the BAC water vial with an alcohol swab Draw 0.5 mL of bacteriostatic water into a 3 mL syringe Inject the BAC water slowly into the MOTS-c vial, angling the stream down the side wall rather than directly onto the powder Gently swirl the vial until the powder is fully dissolved, do not shake Draw 25 units from the reconstituted vial into an insulin syringe for injection Store the reconstituted vial in the refrigerator between doses Injection Site and Technique The injection is subcutaneous, into the abdomen

allow to dry

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Finally, the visualization of the docking results was completed using PyMOL (version 3.9.1)