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After 24 h, the cells were lysed in NP40-lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5 mM sodium orthovanadate, 0.5 mM PMSF, 0.5 mM sodium molybdate, and cOmplete protease inhibitor cocktail)

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Oxidized ascorbate (DHA) can be recycled back to ascorbate (AscH ) by 2-electron reduction catalyzed by dehydroascorbate reductase (DHAR), a key enzyme involved in ascorbate recycling (see Fig

Role of Vitamin B12 in Boosting Immunity Vitamin B12 is intricately linked to immune system function

All tissue biopsies were placed in a drying chamber until a stable weight was measured for approximately 48 hours

Direct control of hepatic glucose production by interleukin-13 in mice