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In a retrospective analysis of ninety-day toxicity study data from eighty-three mouse studies and eighty-seven rat studies that were also tested for carcinogenicity, the combined presence of hepatocellular necrosis, hepatocellular hypertrophy, hepatocellular cytomegaly, bile duct hyperplasia, and hepatocellular degeneration, along with increased liver weight, was significantly predictive of hepatocarcinogenicity in the two-year study (Allen et al

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10.1038/jcbfm.2009.72 J

Kalogeris et al., 2014)

Following the incubation, the mixture was washed five times with PBS buffer containing 0.05% Tween 20