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If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C

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This reduction in T3 can manifest as hypothyroid symptoms such as fatigue, weight gain, and cold intolerance, even though T4 levels may appear normal

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