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For permeabilization, the samples were treated successively with a mixture of Spurr embedding agent and acetone (volume ratio 1:1) for 1 h, a mixture (volume ratio 3:1) for 3 h, and pure Spurr embedding agent overnight

[PubMed: 3790639] 61

Qifan Z, Daniel K, Abel S-F, Sean G, Liang J, Shivesh P et al

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Aberrant DNA methylation in genes regulating immune function, such as FOXP3 and CTLA -4, contributes to altered immune tolerance and disease progression