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MTT solution was added to each well, followed by incubation for 1 h at a final concentration of 5 mg/mL in 5% CO 2 at 37 C

M., Singh S

J Child Neurol (2000) 15(6):35761

TRPM2 channel aggravates CNS inflammation and cognitive impairment via activation of microglia in chronic cerebral hypoperfusion

These nutraceuticals have some mechanisms of action in common, including the inhibition of ACE, the enhancement of NO-mediated vasodilation, interactions with ion channels including the blocking of calcium channels and opening of potassium channels with consequent membrane hyperpolarization, anti-inflammatory effects with reductions in synthesis and release of pro-inflammatory cytokines, and enhancement of endogenous antioxidant actions exerted by different enzymes, such as SOD, GPx, and GSTP

Most gastrointestinal events were classified as mild to moderate, and the majority were transient, resolving within the first several weeks of treatment or after dose stabilization