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Based on the nucleotide sequences of the target genes, development of primer sets were designed and used to amplify gene fragments by PCR (Additional file 1: Tables S1S6)

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Store reconstituted solution at 2-8C and use within 30 days

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however, the two spindles failed to align and come into close apposition to form a compound barrel-shaped structure, producing 2-cell embryos with one or two binucleated blastomeres, which provides a potential rationale for the multinucleated blastomere formation observed in human IVF-derived embryos 22