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Asakura T, Ohkawa K, Takahashi N, Takada K, Inoue T, Yokoyama S

This review will provide the status of APAP protein adducts and systems biology omics biomarkers (miRNAs, protein biomarkers and metabolite biomarkers) of APAP liver injury in the clinical setting

agalactiae [6] is one of the two (other being Listeria monocytogenes [5]) organisms first reported to synthesize GSH by a multidomain bifunctional fusion protein (GshF or GshAB) in which the N-terminal domain is responsible for -glutamylcysteine synthetase activity and the C-terminal domain carries glutathione synthetase activity

The results in the [U- 13 C]-glucose labeling experiment showed the similar conclusion wherein 13 C-labeled metabolites in RPs (45.7%) had higher chances of similar isotopologue patterns than those in non-RPs (14.6%)

After brief staining with Coomassie Brilliant Blue, the bands were excised and stored in 10% acetic acid

sgRNA sequences were verified by Sanger sequencing (Genomed, Warsaw, Poland) Table 1