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To identify POMC neurons and GLP-1 receptor-positive neurons, POMC humanized Renilla green fluorescent protein (hrGFP) (The Jackson Laboratory, 006421) and GLP-1 receptor cre::tdTomato (The Jackson Laboratory, 029283) mice were used

[0590] In certain embodiments the CAR-modified T cells described herein can also serve as a type of vaccine for ex vivo immunization and/or in vivo therapy in a mammal In certain embodiments the mammal is a non-human mammal and in other embodiments the mammal is a human

Li G, Wu R, Tong R, et al

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Neurons were visualized with infrared differential interference contrast imaging and a CCD camera under a water-immersion objective (40 , 0.8 numerical aperture)