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Mitochondria, the tiny powerhouses of our cells, rely on magnesium to generate ATPthe bodys main source of energy

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Reaction buffer was freshly prepared by mixing 2.8 ml of 1 mM 5,5-dithiobis (2-nitro-benzoic acid), 3.75 ml of 1 mM NADPH, 5.85 ml of 100 mM NaH2PO4, 5 mM EDTA phosphate-EDTA buffer, and 20 U of glutathione reductase (Sigma-Aldrich)

The outcomes were quantified and reported in units of CAT activity/mg of protein (U/mg of protein) [30]

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Under most conditions this may be the most important short-term regulatory signal, although it is, of course, possible that other regulatory effects play a role as well