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Cells were counted by a hemocytometer and plated at 0.5 10 6 cells/mL/well in DMEM containing FCS (10%), penicillin-streptomycin (1%), and L929 supernatant (10%) in 12-well plates unless otherwise stated

114 The anorectic effects of GLP-1 may be mediated through NPY signaling since GLP-1 inhibits, and exendin-3 (9-39) promotes NPY-induced feeding

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an effect that is, in part, mediated by impaired NO activity [92,93]

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