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Five days after DSS treatment, the number of active neuronal cells in the DVN, NST, and CeA increased in the brain of the Con-DSS group, revealing that the control mice felt extensive visceral discomfort (Fig

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The NMR spectroscopy in this experiment was conducted at NIST's Gaithersburg, Md., facility in collaboration with NIST scientists at the Hollings Marine Laboratory (HML) in Charleston, S.C., and the Institute for Bioscience and Biotechnology Research (IBBR) in Rockville, Md.

4a, b and Supplementary Fig

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Refrigerate opened vials at 28C, mark the 28-day discard date, and treat temperature excursions as protocol failures