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If sterile water without preservative is used, use within 24-48 hours

Reactions were initiated by the addition of 10 reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl, 50 mM ATP, 5 mM DTT) and incubated at 37 C for the specified durations

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Lesion counts and skin hemoglobin concentrations were evaluated at these assessment periods as well, while cream tolerability was examined at weeks 4 and 8