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Protein oxidation occurs when ROS modifies amino acid side chains, resulting in changes to protein structure and function

[125] also reported significantly increased Kynurenine/Tryptophan ratio in ME/CFS [125], but in contrast to Kavyani et al

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Cell culture and transfection Neuro2A cells were grown in a 1:1 mixture of DMEM and Opti-MEM, supplemented with 5% fetal bovine serum, 100 U/ml penicillin, and 100 g/ml streptomycin