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The cells were incubated in 6-well plates (2 mL/well) at a density of 150,000 per well for 24 h, followed by treatment with test compounds with or without cisplatin (1 M) for 48 h

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Furthermore, disruption in the CR process leads to the production of higher CTCs, particularly during sleep time, which can further promote the tumor progression as these CTCs display elevated expression of circadian hormone receptors, and hormones such as melatonin modulate their release (174, 175)

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Statements Author contributions WL: Conceptualization, Data curation, Formal Analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review and editing

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