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Zile, M

For the ferroptosis inhibitor study, HepG2 cells (1 10 7 cells per mouse) were subcutaneously injected to the left back of male BALB/C nude mice (6-8 weeks old, n = 6, 4 groups)

Z-stack images were obtained on a Zeiss AxioObserverZ1 LSM800 Confocal Microscope at 63 magnification with a 5 digital zoom through a Z distance of 1012 m per image using optimal 0.27 m spacing per slice with dimensions set to 512 512 pixels with 2X averaging per frame

In other series of experiments, higher concentrations of methyl-L-[3H]-carnitine, as well as Na+, and Cl dependence of the transport were tested

I find that different people respond differently to different binders

Receiving an IV can be slightly different for each individual