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10.3969/j.issn.1001-1528.2021.08.043 145 OldsH.LiuJ.LukK.LimH

DNA libraries were PCR-amplified, DNA qualityverified on 2100 Bioanalyzer (Agilent) using the DNA 1000 kit, and size-selected from 200 to 800 bp, following the manufacturers recommendations

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The cells were then incubated with antibodies against arrestin3 or HA (1:1000 dilution) for 1 h at 20 C, followed by washing with PBS and incubation with the secondary antibodies (Alexa 555-conjugated antibodies at 1:500 dilution)

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[33] noted that miR-126 had functional relevance in neurotoxicity