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It is worth noting that dysregulation of DNA methylation patterns has been linked to various brain disorders, including depression, anxiety, and cognitive decline (reviewed in: 14,15 )

Further experiments revealed that hydrogen-induced Nrf2 can directly bind to NF-B p65, interfering with its DNA-binding ability to pro-inflammatory gene promoters while also suppressing the release of inflammatory mediators such as HMGB1 and TNF-, thereby truncating the positive feedback loop of IKK